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Data Sheet IMAC/Ni SepFast HighRes
IMAC/Ni SepFast HighRes is designed and fabricated for high performance chromatographic purification of proteins in packed columns, particularly in FPLC systems. The base matrix is made of heavily cross-linked polysaccharide. Its small spherical shaped particle and carefully controlled pore structure allows superior chromatographic resolution in a packed column operation. Also, its high mechanical strength permits liquid passing through the pressurised column at reasonable flow rates with low to medium pressure drop. All these translate into higher purification performance at shortened purification time.
Metal ions can be charged to the covalently attached chelating groups on the solid support. Leakage of metal ions is negligible.