MCI Gel™ Hydrophobic Interaction Resins & Columns for Protein Analysis and Purification

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The MCI Gel™ hydrophobic interaction columns and resins are based on polyhydroxymethacrylate functionalised with phenyl, butyl or ether. The relative hydrophobicity of these resins is as follows: phenyl > butyl > ether.
The columns are packed with 10 µm bead size and the resins are available with bead sizes of 10 µm and 30 µm.

On this page: List of available columns and resins followed by some sample chromatograms.

CQH-S series hydrophobic interaction chromatography columns

Art. Nr.Product NameDimensions ID x LParticle Size (µm)Functional Group
0216-01MCI Gel™ CQH3BS7.5 mm x 75 mm10Butyl
0217-01MCI Gel™ CQH3ES7.5 mm x 75 mm10Ether
0218-01MCI Gel™ CQH3PS7.5 mm x 75 mm10Phenyl

CQH series hydrophobic interaction chromatography resins

Art. Nr.Product NamePacking SizeParticle Size (µm)Pore Size (nm)Functional Group
1216-01MCI Gel™ CQH3BS101060Butyl
1216-02MCI Gel™ CQH3BS251060Butyl
1216-03MCI Gel™ CQH3BS501060Butyl
1217-01MCI Gel™ CQH3ES101060Ether
1217-02MCI Gel™ CQH3ES251060Ether
1217-03MCI Gel™ CQH3ES501060Ether
1218-01MCI Gel™ CQH3PS101060Phenyl
1218-02MCI Gel™ CQH3PS251060Phenyl
1218-03MCI Gel™ CQH3PS501060Phenyl
1226-01MCI Gel™ CQH3BP253060Butyl
1226-02MCI Gel™ CQH3BP1003060Butyl
1226-03MCI Gel™ CQH3BP1000 ml (!)3060Butyl
1227-01MCI Gel™ CQH3PP253060Phenyl
1227-02MCI Gel™ CQH3PP1003060Phenyl
1227-03MCI Gel™ CQH3PP1000 ml (!)3060Phenyl

Example chromatograms – hydrophobic interaction columns and resins

Mitsubishi MCI Gel™ CQH3ES – Separation of Colibacillus Extract
ColumnMCI Gel™ CQH3ES
(7.5 mm ID x 75 mm)
EluentA B+1.7M (NH4)2SO4
B 0.1M Phosphate buffer
A -> B 30 min. linear gradient
Flow Rate1.0 ml/min
Column TemperatureAmbient
Detection280 nm
SampleColibacillus extract
Mitsubishi MCI Gel™ CQH3ES Separation of Colibacillus Extract
Mitsubishi MCI Gel™ CQH3PS – Separation of Colibacillus Extract
ColumnMCI Gel™ CQH3PS
(7.5 mm ID x 75 mm)
EluentA B+1.7M (NH4)2SO4
B 0.1M Phosphate buffer
A -> B 30 min. linear gradient
Flow Rate1.0 ml/min
Column TemperatureAmbient
Detection280 nm
SampleColibacillus extract
Mitsubishi MCI Gel™ CQH3PS Separation of Colibacillus Extract
Mitsubishi MCI Gel™ CQH3PS – Separation of Mixture of Peptides
ColumnMCI Gel™ CQH3PS
(7.5 mm ID x 75 mm)
EluentA B+1.7M (NH4)2SO4
B 0.1M Phosphate buffer pH 6.8
A -> B 30 min. linear gradient
Flow Rate1.0 ml/min
Column TemperatureAmbient
Detection220 nm
Sample1. Met-Leu-Tyr
2. Leu-Enkephalin
3. Bacitracin
Mitsubishi MCI Gel™ CQH3PS Separation of Mixture of Peptides
Mitsubishi MCI Gel™ CQH3BS & CQH3BP – Proteins
ColumnMCI Gel™ CQH3BS
MCI Gel™ CQH3BP
(7.5 mm ID x 75 mm)
EluentA B+1.7M (NH4)2SO4
B 0.1M Phosphate buffer pH 6.8
GradientA -> B 30 min. linear
Flow Rate1.0 ml/min
Column Temperature25 ºC
Detection280 nm
Sample1. Ribonuclease A, 112 µg
2. Transferrin, 154 µg
3. α-Chymotrypshinogen A, 60 µg
Mitsubishi MCI Gel™ CQH3BS Proteins
10 µm MCI Gel™ CQH3BS
Mitsubishi MCI Gel™ CQH3BP Proteins
30 µm MCI Gel™ CQH3BP
Mitsubishi MCI Gel™ CQH3PS & CQH3PP – Proteins
ColumnMCI Gel™ CQH3PS
MCI Gel™ CQH3PP
(7.5 mm ID x 75 mm)
EluentA B+1.7M (NH4)2SO4
B 0.1M Phosphate buffer pH 6.8
GradientA -> B 30 min. linear
Flow Rate1.0 ml/min
Column Temperature25 ºC
Detection280 nm
Sample1. Ribonuclease A, 112 µg
2. Transferrin, 154 µg
3. α-Chymotrypshinogen A, 60 µg
Mitsubishi MCI Gel™ CQH3PS Proteins
10 µm MCI Gel™ CQH3PS
Mitsubishi MCI Gel™ CQH3PP Proteins
30 µm MCI Gel™ CQH3PP

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MCI Gel™ Hydrophobic Interaction Resins & Columns for Protein Analysis and Purification

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