Sykam S433 Amino Acid Analyzer hydrolysate standards and hydrolysate analysis

Shown below, some sample chromatograms of the routine hydrolysate and oxidized hydrolysate analysis programs.
The graphs are 700×400 dots. Zooming in could reveal more detail on your monitor. pdf-files with some additional examples are available on the left side of this page or in the download section of this website.

Fig.1. Standard Hydrolysate analysis program. Retention time to Arginine 42 minutes. Injection to injection time 58 minutes.
Fig. 2. Standard Oxidized Hydrolysate analysis program. Retention time to Arginine 46 minutes. Injection to injection time 59 minutes.
Fig.3. Oxidized hydrolysate program based on Lithium buffer system. Enabling to run hydrolysate and physiological programs without changing any buffers or columns. Retention time to Arginine 57 minutes. Injection to Injection time 77 minutes.
Fig.4. Oxidized hydrolysate program, showing 440nm channel for Hydroxyproline and proline. Additional amino acid ß-Alanine eluting after Phenylalanine. Retention time to Arginine 68 minutes. Injection to injection time 88 minutes. 
Fig.5. Same as Fig.4. showing Proline, Glycine, Alanine, Cystine, Valine, Methionine, Isoleucine, Leucine, Norleucine, Tyrosine, Phenylalanine, ß-Alanine.
Fig.6. Same as Fig.4. showing 440nm channel mid section of chromatogram. Illustrating baseline quality with low drift and without any “buffer steps!”. Without baseline substraction or baseline smoothing!
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