HiSep CM SepFast 6HF IEX Column

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The HiSep CM SepFast 6HF column belongs to a group of weak cation exchange media, designed for the general purification of biological molecules in which impure components can be chromatographically separated from target molecules at a less demanding process.

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Description

The HiSep CM SepFast 6HF column belongs to a group of weak cation exchange media.

The above ion-exchange chromatography (IEX) media is designed for the general purification of biological molecules in which impure components can be chromatographically separated from target molecules at a less demanding process.

The base matrix is made of highly cross-linked 6% agarose. It is very stable to most of the chemical conditions experienced in the bioprocessing industry. The IEX SepFast 6HF could be a good alternative to the IEX Sepharose 6FF with the benefit of reduced cost. We provide the regulatory support files of individual resins for those cGMP applications.

The core advantages to large-scale biomanufacturings are:

  • Good dynamic binding capacity
  • Good pressure / flow property
  • Low cost

Additional information

Weight N/A
Dimensions N/A
Class

Volume

5 x 1 ml, 5 x 5 ml

Matrix

Highly cross-linked 6% agarose

Functional group

Total ionic capacity

0.08 – 0.13 mmol/ml

Binding capacity

>110 mg lysozyme / ml

Particle size

Pressure-flow property

>500 cm/h

Operational pressure

Up to 3 bar

pH stability

2 – 14 (short term), 3 – 12 (long term)

Working temperature

4 ºC – 30 ºC

Chemical stability

All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol

Avoid

Cationic detergents, Oxidizing agents

Storage

20% ethanol

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