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The HiSep Q SepFast HighRes column belongs to a group of strong anion ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.
Description
The HiSep Q SepFast HighRes column belongs to a group of ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.
The core advantages are:
- High resolution at high loading
- High sample loading capacity at high flow
- High productivity
The base matrix of IEX SepFast HighRes is made of highly cross-linked agarose. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.
Additional information
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Class | |
| Volume | 5 x 1 ml, 5 x 5 ml |
| Matrix | Highly cross-linked agarose |
| Total ionic capacity | 0.09 – 0.15 mmol/ml |
| Particle size | |
| Binding capacity | >100 mg/ml BSA |
| Pressure-flow property | 30 – 150 cm/hr is recommended |
| Operational pressure | Up to 3 bar |
| pH stability | 2 – 14 (short term), 3 – 12 (long term) |
| Working temperature | 4 ºC – 30 ºC |
| Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol |
| Avoid | Anionic detergents, Oxidizing agents |
| Storage | 20% ethanol |
