HiSep SP SepFast HighRes column

 154,00 299,00

The HiSep SP SepFast HighRes column belongs to a group of strong cation ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.

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BioToolomics

Description

The HiSep SP SepFast HighRes column belongs to a group of strong cation ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.

The core advantages are:

  • High resolution at high loading
  • High sample loading capacity at high flow
  • High productivity

The base matrix of IEX SepFast HighRes is made of highly cross-linked agarose. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

Additional information

WeightN/A
DimensionsN/A
Class

Volume

5 x 1 ml, 5 x 5 ml

Matrix

Highly cross-linked agarose

Functional group

Total ionic capacity

0.09 – 0.15 mmol/ml

Particle size

Binding capacity

>20 mg/ml hIgG

Pressure-flow property

30 – 150 cm/hr is recommended

Operational pressure

Up to 3 bar

pH stability

2 – 14 (short term), 3 – 12 (long term)

Working temperature

4 ºC – 30 ºC

Chemical stability

All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol

Avoid

Cationic detergents, Oxidizing agents

Storage

20% ethanol + 0.2M sodium acetate

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HiSep SP SepFast HighRes column
 154,00 299,00