HiSep SP SepFast HighRes Plus IEX Column

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The HiSep SP SepFast HighRes Plus column belongs to a group of strong cation ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution and high binding capacity purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.

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Description

The HiSep SP SepFast HighRes Plus column belongs to a group of strong cation ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution and high binding capacity purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.

The core advantages are:

  • High binding capacity
  • High resolution
  • High sample loading capacity at high flow
  • High productivity

IEX SepFast HighRes Plus is made of a composite of agarose and dextran to give increased dynamic binding capacities. The base matrix is highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.

Additional information

Weight N/A
Dimensions N/A
Class

Volume

5 x 1 ml, 5 x 5 ml

Matrix

Highly cross-linked agarose grafted with dextran chain

Functional group

Total ionic capacity

0.09 – 0.15 mmol/ml

Particle size

Binding capacity

>60 mg/ml hIgG

Pressure-flow property

30 – 150 cm/hr is recommended

Operational pressure

Up to 3 bar

pH stability

2 – 14 (short term), 3 – 12 (long term)

Working temperature

4 ºC – 30 ºC

Chemical stability

All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol

Avoid

Cationic detergents, Oxidizing agents

Storage

20% ethanol + 0.2M sodium acetate

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