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The Optio Q SepFast Large Bead Plus column (16 x 100 mm) belongs to a group of strong anion ion exchange chromatography media with large particle sizes (100 – 350 μm) and high binding capacity. The base matrix shows excellent flow property and stable physical property, particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.
Description
The Optio Q SepFast Large Bead Plus column (16 x 100 mm) belongs to a group of strong anion ion exchange chromatography media with large particle sizes (100 – 350 μm) and high binding capacity. The base matrix shows excellent flow property and stable physical property.
The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.
This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications. IEX SepFast Large Beads Plus offers increased binding capacity and faster binding kinetics than IEX SepFast Large Beads.
The SepFast Large Beads Plus is made of highly cross-linked agarose grafted with dextran for increased binding capacity. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.
Additional information
| Weight | 0,2 kg |
|---|---|
| Dimensions | 30 × 10 × 8 cm |
| Class | |
| Volume | 5 ml |
| Matrix | Highly cross-linked agarose and dextran |
| Functional group | |
| Total ionic capacity | 0.1 – 0.2 mmol/ml |
| Particle size | |
| Pressure-flow property | >2000 cm/h |
| Operational pressure | Up to 3 bar |
| pH stability | 2 – 14 (short term), 3 – 12 (long term) |
| Working temperature | 4 ºC – 30 ºC |
| Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol |
| Avoid | Anionic detergents, Oxidizing agents |
| Storage | 20% ethanol |
