Protein A SepFast HighRes Immunoaffinity Resin

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Protein A SepFast HighRes has a particle size of 20 – 50 µm and is good for small affinity chromatography column run at shorter residence time (i.e. higher flowrate).

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Description

Protein A SepFast HighRes has a particle size of 20 – 50 µm, good for small column run at shorter residence time (i.e. higher flowrate).

Protein A SepFast is a family of affinity chromatography media for the purification of immunoglobulins. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.

Standard protein A molecule or more alkaline-stable protein A is immobilised to highly porous and highly cross-linked agarose base matrices. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The medium shows high mechanical rigidity, so it can be operated at moderate to high flow velocities with moderate pressure drop.

We offer both loose media and pre-packed ready-to-use disposable columns for small scale sample preparation and large-scale bioprocessing use.

The range of Protein A SepFast media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.

Additional information

Weight N/A
Dimensions N/A
Class

Volume

5 ml, 25 ml, 100 ml, 250 ml, 500 ml, 1 litre, 5 litre, 10 litre

Matrix

Highly cross-linked agarose beads

Particle size

Operational pressure

Up to 3 bar

Pressure-flow property

50 – 300 cm/hr is recommended

Binding capacity

>30 mg human IgG/mL

Chemical stability

Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11

pH stability

2 – 10 (short term), 3 – 9 (long term)

Working temperature

4 ºC – 30 ºC

Storage

20% ethanol

Brands

Supplying a wide range of chromatography media, industrial ion exchangers & adsorption resins

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Designing, developing and manufacturing of innovative process chromatography media & columns

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Analytical & preparative HPLC systems & valves, ion chromatography systems, dedicated amino acid analyzers

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Development of specialty silica gels for many industrial liquid chromatography applications

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Industrial purification using chromatography, electrodialysis, membrane filtration & crystallization

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BioToolomics Bottle 25ml, Round
Protein A SepFast HighRes Immunoaffinity Resin
 551,60 2.110,76