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The Protein A SepFast HiSep column has a particle size of 20 – 50 µm and is good for most applications from small scale to large scale purification requirements (i.e. low column pressure).
Description
The Protein A SepFast HiSep column has a particle size of 50 – 150 µm, good for most applications from small scale to large scale purification requirements (i.e. low column pressure).
Protein A SepFast is a family of affinity chromatography media for the purification of immunoglobulins. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Standard protein A molecule or more alkaline-stable protein A is immobilised to highly porous and highly cross-linked agarose base matrices. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The medium shows high mechanical rigidity, so it can be operated at moderate to high flow velocities with moderate pressure drop.
We offer both loose media and pre-packed ready-to-use disposable columns for small scale sample preparation and large-scale bioprocessing use.
The range of Protein A SepFast media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.
Additional information
| Class | |
|---|---|
| Volume | 1 ml, 5 ml |
| Matrix | Highly cross-linked agarose beads |
| Particle size | |
| Operational pressure | Up to 3 bar |
| Pressure-flow property | 50 – 300 cm/hr is recommended |
| Binding capacity | >30 mg human IgG/mL |
| Chemical stability | Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11 |
| pH stability | 2 – 10 (short term), 3 – 9 (long term) |
| Storage | 20% ethanol |
| Working temperature | 4 ºC – 30 ºC |
