Protein A SepFast Large Bead

 443,00 1.759,00

Protein A SepFast Large Bead has a particle size of 150 – 350 µm, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure).

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BioToolomics

Description

Protein A SepFast Large Bead has a particle size of 150 – 350 µm, particularly good for processing viscous or cell-containing feedstocks (i.e. high settling rate and extremely low column pressure).

Protein A SepFast is a family of affinity chromatography media for the purification of immunoglobulins. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.

Standard protein A molecule or more alkaline-stable protein A is immobilised to highly porous and highly cross-linked agarose base matrices. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The medium shows high mechanical rigidity, so it can be operated at moderate to high flow velocities with moderate pressure drop.

We offer both loose media and pre-packed ready-to-use disposable columns for small scale sample preparation and large-scale bioprocessing use.

The range of Protein A SepFast media is developed and supported for production scale chromatography use. Regulatory Support File (RSF) is available to assist process validation and submissions to regulatory authorities.

Additional information

WeightN/A
DimensionsN/A
Class

Volume

5 ml, 25 ml, 100 ml, 250 ml, 500 ml, 1 litre, 5 litre, 10 litre

Matrix

Highly cross-linked agarose beads

Particle size

Operational pressure

Up to 3 bar

Pressure-flow property

50 – 300 cm/hr is recommended

Binding capacity

>30 mg human IgG/mL

Chemical stability

Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11

pH stability

2 – 10 (short term), 3 – 9 (long term)

Storage

20% ethanol

Working temperature

4 ºC – 30 ºC

BioToolomics Bottle 25ml, Square
Protein A SepFast Large Bead
 443,00 1.759,00