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SP SepFast Large Bead belongs to a group of strong cation ion exchange chromatography media with large particle sizes (100 – 350 μm). The base matrix shows excellent flow property and stable physical property, particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.
Description
SP SepFast Large Bead belongs to a group of strong cation ion exchange chromatography media with large particle sizes (100 – 350 μm). The base matrix shows excellent flow property and stable physical property.
The above ion-exchange chromatography media is particularly suitable for large-scale purification of biological molecules from viscous and/or crude culture broths or for high speed flow-through polishing applications in which impure components are chromatographically adsorbed from the main product stream.
This group of ion-exchange media has a balanced design among ligand density, loading capacity and separation power of individual components for large-scale biomanufacturing applications.
The base matrix of SepFast Large Beads is made of highly cross-linked agarose with excellent flow property. They are very stable to most of the chemical conditions experienced in the bioprocessing industry.
Additional information
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Class | |
| Volume | 25 ml, 100 ml, 500 ml, 1 litre, 5 litre, 10 litre, 50 litre, 100 litre, 500 litre, 1000 litre |
| Matrix | Highly cross-linked agarose beads |
| Functional group | |
| Total ionic capacity | 0.09 – 0.15 mmol/ml |
| Particle size | |
| Pressure-flow property | >2000 cm/h |
| Operational pressure | Up to 3 bar |
| pH stability | 2 – 14 (short term), 3 – 12 (long term) |
| Working temperature | 4 ºC – 30 ºC |
| Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol |
| Avoid | Cationic detergents, Oxidizing agents |
| Storage | 20% ethanol + 0.2M sodium acetate |
